Anti-Wnt11 antibody

• Supplier: Abcam
• Catalog: ab31962
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Rat, Human, Mouse, Rabbit, Bovine
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Wnt11
• Description: Rabbit Polyclonal
• Gene: WNT11
• Conjugate: Unconjugated

Product images

Anti-Wnt11 antibody (ab31962) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 15 µgSecondaryGoat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

ICC/IF image of ab31962 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31962 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Activation of Wnt11 by transforming growth factor-beta drives mesenchymal gene - Activation of Wnt11 by transforming growth factor-beta drives mesenchymal gene

Zhang P, Cai Y, Soofi A, Dressler GR. J Biol Chem. 2012 Jun 15;287(25):21290-302.

MiR-137 targets estrogen-related receptor alpha and impairs the proliferative and - MiR-137 targets estrogen-related receptor alpha and impairs the proliferative and

Zhao Y, Li Y, Lou G, Zhao L, Xu Z, Zhang Y, He F. PLoS One. 2012;7(6):e39102.

Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into - Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into

He Z, Li H, Zuo S, Pasha Z, Wang Y, Yang Y, Jiang W, Ashraf M, Xu M. Stem Cells Dev. 2011 Oct;20(10):1771-8.

WNT11 expression is induced by estrogen-related receptor alpha and beta-catenin - WNT11 expression is induced by estrogen-related receptor alpha and beta-catenin

Dwyer MA, Joseph JD, Wade HE, Eaton ML, Kunder RS, Kazmin D, Chang CY, McDonnell DP. Cancer Res. 2010 Nov 15;70(22):9298-308.

Wnt-11 signalling controls ventricular myocardium development by patterning - Wnt-11 signalling controls ventricular myocardium development by patterning

Nagy II, Railo A, Rapila R, Hast T, Sormunen R, Tavi P, Rasanen J, Vainio SJ. Cardiovasc Res. 2010 Jan 1;85(1):100-9.