Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µgSecondaryHRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µgSecondaryHRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified)Lane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLane 3 : SKBR-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysates Lane 2 : MCF-7 cell lysates Lane 3 : T47-D cell lysates Lane 4 : SKBR-3 cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution