All lanes : Anti-Wnt2b antibody [EPR13386] (ab178418) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : HepG2 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of HepG2 cells labeling Wnt2b with ab178418 at 1/50 dilution (green). DAPI nuclear staining (blue).
Flow Cytometric analysis of permeabilized Jurkat cells labeling Wnt2b with ab178418 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).