Anti-Wnt3a antibody
| Name | Anti-Wnt3a antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28472 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IP IHC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Chicken, Human, Zebrafish, Xenopus, Broad species |
| Antigen | Synthetic peptide derived from within residues 250 - 350 of Human Wnt3a |
| Blocking Peptide | Human Wnt3a peptide |
| Description | Rabbit Polyclonal |
| Gene | WNT3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab28472 staining Wnt3a in the dentate gyrus of rat bain tissue by Immunohistochemistry (Frozen sections).Brains were sectioned in the coronal plane at 20 µm. The slides were left in a fume hood to dry overnight, then stored at -70°C. For each rat twelve sections through the dorsal hippocampus were analyzed. For single staining with Wnt3a, sections were incubated with ab28472 at 5 ug/ml in 0.5% Triton with 1% goat serum) overnight in a humidified chamber at room temperature. The following day, after two washes with KPBS, they were incubated in Alexa Fluor 568 goat anti-rabbit (1/200 in 0.5% Triton) for one hour, and washed twice with KPBS and covered slipped.
IHC-Wholemount image of Wnt3a (ab28472) on zebrafish larvae. Whole zebrafish larvae were fixed in paraformaldehyde and then permeabilized in proteinase K, before blocking in 5% serum for 1 hour at RT. The image above is a ventral view of the head, showing the ceratohyal cartilage (of the jaw).See Abreview
Product References
Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine. - Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine.
Gao X, Hannoush RN. Nat Chem Biol. 2014 Jan;10(1):61-8.
IUGR disrupts the PPARgamma-Setd8-H4K20me(1) and Wnt signaling pathways in the - IUGR disrupts the PPARgamma-Setd8-H4K20me(1) and Wnt signaling pathways in the
Ke X, Xing B, Yu B, Yu X, Majnik A, Cohen S, Lane R, Joss-Moore L. Int J Dev Neurosci. 2014 Nov;38:59-67.
Single-cell in situ imaging of palmitoylation in fatty-acylated proteins. - Single-cell in situ imaging of palmitoylation in fatty-acylated proteins.
Gao X, Hannoush RN. Nat Protoc. 2014 Nov;9(11):2607-23.
Deletion of Porcn in mice leads to multiple developmental defects and models - Deletion of Porcn in mice leads to multiple developmental defects and models
Liu W, Shaver TM, Balasa A, Ljungberg MC, Wang X, Wen S, Nguyen H, Van den Veyver IB. PLoS One. 2012;7(3):e32331.
Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in
Mattes B, Weber S, Peres J, Chen Q, Davidson G, Houart C, Scholpp S. Neural Dev. 2012 Apr 4;7:12.
Novel control by the CA3 region of the hippocampus on neurogenesis in the dentate - Novel control by the CA3 region of the hippocampus on neurogenesis in the dentate
Liu JX, Pinnock SB, Herbert J. PLoS One. 2011 Mar 18;6(3):e17562.
Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst - Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst
Gerhart J, Scheinfeld VL, Milito T, Pfautz J, Neely C, Fisher-Vance D, Sutter K, Crawford M, Knudsen K, George-Weinstein M. Dev Biol. 2011 Nov 1;359(1):12-25.
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes. - Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes.
Gao X, Arenas-Ramirez N, Scales SJ, Hannoush RN. FEBS Lett. 2011 Aug 4;585(15):2501-6.
The roles of BDNF, pCREB and Wnt3a in the latent period preceding activation of - The roles of BDNF, pCREB and Wnt3a in the latent period preceding activation of
Pinnock SB, Blake AM, Platt NJ, Herbert J. PLoS One. 2010 Oct 27;5(10):e13652.
Tumor hypoxia blocks Wnt processing and secretion through the induction of - Tumor hypoxia blocks Wnt processing and secretion through the induction of
Verras M, Papandreou I, Lim AL, Denko NC. Mol Cell Biol. 2008 Dec;28(23):7212-24.