Anti-Wnt3a antibody
| Name | Anti-Wnt3a antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19925 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Xenopus, Chicken, Human, Zebrafish, Broad species |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Wnt3a |
| Description | Rabbit Polyclonal |
| Gene | WNT3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Wnt3a staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19925, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
Anti-Wnt3a antibody (ab19925) at 1 µg/ml + Recombinant mouse Wnt3a, AAs 19-352 with a 16 AA n-terminal non-Wnt3a sequence at 0.1 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/15000 dilution
ICC/IF image of ab19925 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19925, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
High glucose induces podocyte injury via enhanced (pro)renin - High glucose induces podocyte injury via enhanced (pro)renin
Li C, Siragy HM. PLoS One. 2014 Feb 12;9(2):e89233.
Activation of the transcription factor GLI1 by WNT signaling underlies the role - Activation of the transcription factor GLI1 by WNT signaling underlies the role
Nakamura I, Fernandez-Barrena MG, Ortiz-Ruiz MC, Almada LL, Hu C, Elsawa SF, Mills LD, Romecin PA, Gulaid KH, Moser CD, Han JJ, Vrabel A, Hanse EA, Akogyeram NA, Albrecht JH, Monga SP, Sanderson SO, Prieto J, Roberts LR, Fernandez-Zapico ME. J Biol Chem. 2013 Jul 19;288(29):21389-98.
Region-specific contribution of ephrin-B and Wnt signaling to receptive field - Region-specific contribution of ephrin-B and Wnt signaling to receptive field
Lim BK, Cho SJ, Sumbre G, Poo MM. Neuron. 2010 Mar 25;65(6):899-911.
Early cell fate decisions of human embryonic stem cells and mouse epiblast stem - Early cell fate decisions of human embryonic stem cells and mouse epiblast stem
Vallier L, Touboul T, Chng Z, Brimpari M, Hannan N, Millan E, Smithers LE, Trotter M, Rugg-Gunn P, Weber A, Pedersen RA. PLoS One. 2009 Jun 30;4(6):e6082.