Anti-Wnt6 antibody
| Name | Anti-Wnt6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab50030 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Wnt6 |
| Description | Rabbit Polyclonal |
| Gene | WNT6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Wnt6 antibody (ab50030) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 Human embryonic kidney cell line Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab50030 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50030, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
WNT6 is a novel target gene of caveolin-1 promoting chemoresistance to epirubicin - WNT6 is a novel target gene of caveolin-1 promoting chemoresistance to epirubicin
Yuan G, Regel I, Lian F, Friedrich T, Hitkova I, Hofheinz RD, Strobel P, Langer R, Keller G, Rocken C, Zimmermann W, Schmid RM, Ebert MP, Burgermeister E. Oncogene. 2013 Jan 17;32(3):375-87.
Effect of Wnt6 on human dental papilla cells in vitro. - Effect of Wnt6 on human dental papilla cells in vitro.
Wang C, Ren L, Peng L, Xu P, Dong G, Ye L. J Endod. 2010 Feb;36(2):238-43.