Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate)Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)developed using the ECL technique