Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram) labeling ZDHHC2 with ab174967 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue labeling ZDHHC2 with ab174967 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
All lanes : Anti-ZDHHC2 antibody - N-terminal (ab174967) at 1/60 dilutionLane 1 : Ramos cell lysateLane 2 : Ramos cell lysate with Blocking peptide
Anti-ZDHHC2 antibody - N-terminal (ab174967) at 1/100 dilution + Neuro-2a cell lysate at 15 µg