All lanes : Anti-ZFAND2B antibody [3A5] (ab118401) at 1/2000 dilutionLane 1 : Lysate of HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : Lysate of HEK293T cells transfected with pCMV6-ENTRY ZFAND2B cDNALysates/proteins at 5 µg per lane.
HEK293T cells transfected with either ZFAND2B overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained by ab118401 at 1/100, and then analyzed by flow cytometry.
Flow cytometric analysis of Hela cells, using ab118401 at 1/100 (red), compared to a nonspecific negative control antibody (blue).
Flow cytometric analysis of Jurkat cells, using ab118401 ab1/100 (red), compared to a nonspecific negative control antibody (blue).