Flow cytometry analysis of 2% paraformaldehyde fixed HepG2 cells labeling ZFY with ab185541 at 1/220 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-ZFY antibody [EPR11074] (ab185541) at 1/5000 dilutionLane 1 : 293T cell lysateLane 2 : A549 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-ZFY antibody [EPR11074] (ab185541) at 1/1000 dilutionLane 1 : Jurkat lysateLane 2 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution