Anti-Zinc Alpha 2 Glycoprotein antibody
| Name | Anti-Zinc Alpha 2 Glycoprotein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47116 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB |
| Species Reactivities | Human |
| Antigen | Recombinant human Zinc Alpha 2 Glycoprotein |
| Description | Rabbit Polyclonal |
| Gene | AZGP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab38929 (4µg/ml) staining MMP1 - Hemopexin domain in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of epithelial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab47116) at 1 µg/mlLane 1 : Human Plasma Total Protein LysateLane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Investigation of ovarian cancer associated sialylation changes in N-linked - Investigation of ovarian cancer associated sialylation changes in N-linked
Shetty V, Hafner J, Shah P, Nickens Z, Philip R. Clin Proteomics. 2012 Aug 2;9(1):10.
Tandem 18O stable isotope labeling for quantification of N-glycoproteome. - Tandem 18O stable isotope labeling for quantification of N-glycoproteome.
Liu Z, Cao J, He Y, Qiao L, Xu C, Lu H, Yang P. J Proteome Res. 2010 Jan;9(1):227-36.