Immunofluorescence of MDA-MB231 cells labelling ZNF202 with ab103034. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103034 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF202 immunoreactivity is localized to the nucleus.
Immunoflurescence of HepG2 cells labelling ZNF202 with ab103034. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).
Flow cytometry of HepG2 cells (right histogram) labelling ZNF202 with ab103034 compared to a negative control (left histogram). FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
Anti-ZNF202 antibody (ab103034) at 1/100 dilution + HepG2 cell line lysates at 35 µg
ab103034, at a 1/50 dilution, staining ZNF202 in formalin fixed and paraffin embedded Human breast carcinoma, followed by peroxidase conjugation of the secondary antibody and DAB staining.