Western blot analysis of extracts from Drosophila S2 cells, untreated (-) or treated with either cycloheximide (10 μM, 5 hr; +) or actinomycin D (0.7 μM, 5 hr; +), using Cleaved Drosophila Dcp-1 (Asp216) Antibody.
Confocal immunofluorescent analysis of S2 cells, untreated (left) or treated with Actinomycin D (0.7 uM, 20 hr; right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of wild-type (white 1118 ) Drosophila egg chambers, conditioned (left) or conditioned followed by starvation (right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).