Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ZNF24 with ab180121 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
All lanes : Anti-ZNF24 antibody [EPR13975] (ab180121) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution