Anti-ZNF312 antibody
| Name | Anti-ZNF312 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69436 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse ZNF312 |
| Description | Rabbit Polyclonal |
| Gene | FEZF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-ZNF312 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab69436 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.