Anti-ZNF35 antibody (ab168126) at 1/500 dilution + Human ovarian cancer lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-ZNF35 antibody (ab168126) at 1/500 dilutionLane 1 : ZNF35 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of HeLa cells labeling ZNF35 with purified ab168126 at 10 µg/ml.Antibody purification with Protein A will be needed prior to use.