Anti-ZNF468 antibody (ab68014) at 1/500 dilution + human pancreas tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 55 kDa (why is the actual band size different from the predicted?)Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-ZNF468 antibody (ab68014) at 1/500 dilutionLane 1 : ZNF468 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 65 kDa (why is the actual band size different from the predicted?)