Western Blot analysis of ACYP2 expression in transfected 293T cell line by ACYP2 monoclonal antibody (M06), clone 2H7.Lane 1: ACYP2 transfected lysate(11.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ACYP2 is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of ACYP2 over-expressed 293 cell line, cotransfected with ACYP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ACYP2 monoclonal antibody (M06), clone 2H7 . GAPDH ( 36.1 kDa ) used as specificity and loading control.