Detection of Human TANC2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.
Antibodies: Affinity purified rabbit anti-TANC2 antibody A303-023A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TANC2 was also immunoprecipitated by rabbit anti-TANC2 antibody A303-022A, which recognizes an upstream epitope.
Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).