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Immunofluorescence staining of Mouse PVR(m CD155) in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse PVR (m CD155) monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
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Flow cytometric analysis of Mouse PVR(CD155) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse PVR(CD155), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.