Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after protein transfer.
Confocal immunofluorescent analysis of postnatal day 5 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of P19 differentiated cells using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).