
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALDH1A3 mouse monoclonal antibody.

IHC of paraffin-embedded Human prostate tissue using anti-ALDH1A3 mouse monoclonal antibody.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ALDH1A3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human ALDH1A3 (NP_000648) gene (the fusion of amino acids 1-100 and 413-512) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted with anti-ALDH1A3. .

Flow cytometry of Jurkat cells, using anti-ALDH1A3 antibody (Red), compared to a nonspecific negative control antibody (Blue).