Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kD.
Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kD band corresponding to human APC10 (predicted molecular weight is 21 kD). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B327 concentration 5 ug/ml.