alpha Adaptin Monoclonal Antibody (AP6)
| Name | alpha Adaptin Monoclonal Antibody (AP6) |
|---|---|
| Supplier | Thermo Fisher Scientific |
| Catalog | MA1-064 |
| Prices | $324.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AP6 |
| Applications | FC B/N ICC/IF IHC-P IP WB |
| Species Reactivities | Bovine, Hamster, Human, Mouse, Primate, Rat |
| Antigen | Purified adaptors. |
| Purity/Format | purified |
| Description | Mouse Monoclonal |
| Gene | AP2A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
alpha Adaptin Antibody (MA1-064)
Immunofluorescence analysis of alpha Adaptin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Adaptin (AP6) Mouse Monoclonal Antibody (MA1064) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing punctuated cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.