EAF2 (E1R8C) Rabbit mAb
| Name | EAF2 (E1R8C) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 14159 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E1R8C |
| Applications | WB IP ChIP |
| Species Reactivities | Human |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human EAF2 protein. |
| Description | Rabbit Monoclonal |
| Gene | EAF2 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from RL-7 and 293T cell lines using EAF2 (E1R8C) Rabbit mAb.
Immunoprecipitation of EAF2 from RL-7 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or EAF2 (E1R8C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EAF2 (E1R8C) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells heat shocked for 1 hr and either 10 µl of EAF2 (E1R8C) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human c-Myc intron 1 primers, SimpleChIP ® Human HSP70 Intron 1 Primers #14023, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.