RelB (C-19): sc-226. Western blot analysis of RelB p68 expression in KNRK nuclear extract.
ChIP analysis of cytokine gene promoter occupancy in TNFα-treated mouse embryonic fibroblasts. IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): sc-109, RelB (C-19): sc-226 and p300 (N-15): sc-584. Data kindly provided by A. Hoffmann.
RelB (C-19): sc-226. Western blot analysis of RelB expression in non-transfected: sc-117752 (A) and human RelB transfected: sc-114651 (B) 293T whole cell lysates.
RelB (C-19): sc-226. Western blot analysis of RelB expression in non-transfected 293T: sc-117752 (A), mouse RelB transfected 293T: sc-127459 (B) and KNRK2 (C) whole cell lysates.
RelB (C-19): sc-226. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
RelB (C-19): sc-226. Quantitative RT-PCR data using primers spanning the promoter of the IkBa gene (Nfkbia, a known NFkB target) of ChIP samples prepared from murine splenic B-cells stimulated with 50ng/ml BAFF for indicated times. 5g of antibody and 107 cells were used for each sample. The specificity of the signal is confirmed by using mock IgG (sc-2025) and primers for an unrelated locus where NFkB does not bind (Not shown). Kindly provided by the Dr. Alexander Hoffmann Laboratory, University of California San Diego.