Formalin-fixed and paraffin-embedded human brain tissue reacted with ARF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2).
Western blot analysis of ARF6 Antibody (C-term) in T47D,MCF-7 cell line lysates (35 ug/lane). ARF6 (arrow) was detected using the purified Pab.(2 ug/ml)
ARF6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.