EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 2085
• Prices: $359.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D6B6
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.
• Description: Rabbit Monoclonal
• Gene: EGFR

Product images

Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (upper), and total EGFR control antibody (lower).

Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (upper) and total EGFR (D38B1) XP ® Rabbit mAb #4267 (lower).

Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (upper) and total EGFR (D38B1) XP ® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).

Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (upper, green) and EGFR (D38B1) XP ® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (D6B6) XP ® Rabbit mAb.