Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain using EGR1 (15F7) Rabbit mAb in the presence of control peptide (left) or EGR1 (15F7) Blocking Peptide #1015 (right).
Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embeded human Non-Hodgkin's lymphoma, using EGR1 (15F7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb.
Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (15F7) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (15F7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.