Western Blot analysis of ATF3 expression in transfected 293T cell line by ATF3 monoclonal antibody (M01), clone 6B8.Lane 1: ATF3 transfected lysate(20.6 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ATF3 is approximately 1 ng/ml as a capture antibody.
Western blot analysis of ATF3 over-expressed 293 cell line, cotransfected with ATF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF3 monoclonal antibody (M01), clone 6B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.