Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb (upper) or eIF2alpha Antibody #9722 (lower).
Western blot analysis of extracts from HeLa and THP-1 cells untreated or phosphatase treated, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.
Western blot analysis of immunoprecipitates from PC12 cells, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. Lane 1 is lysate control, lane 2 is the antibody alone as negative control and lane 3 is antibody immunocomplex of PC12 cells.
Immunohistochemical analysis of paraffin-embedded PC-3 cells untreated (left) or thapsigargin-treated (right), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb in the presence of control peptide (left) or Phospho-eIF2alpha (Ser51) Blocking Peptide (#1221) (right).