Anti-ATG4D Antibody (aa49-79)
| Name | Anti-ATG4D Antibody (aa49-79) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C156602 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | ATG4D antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human ATG4D. |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | ATG4D Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ATG4D |
| Supplier Page | Shop |
Product images
ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent image of U251 cells stained with ATG4D antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4D primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ATG4D Antibody western blot of mouse liver tissue lysates (35 ug/lane). The ATG4D antibody detected the ATG4D protein (arrow).