Anti-ATP5O antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunofluorescence of ATP5O Antibody with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Western blot of ATP5O (arrow) using rabbit polyclonal ATP5O Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.
Western blot of ATP5O Antibody in 293 cell line lysates (35 ug/lane). ATP5O (arrow) was detected using the purified antibody.
Flow cytometric of 293 cells using ATP5O Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.