Western blot of cell lysates using V-ATPase Subunit B 2 Antibody, pAb, Rabbit (LS-C51666, 1 ug/ml) The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit Predicted Size: 56 kD Observed Size: 58 kD