Western blot analysis of extracts from 293 cells expressing GST-eIF4GI Ser1192Ala or GST-eIF4GI Ser1108Ala mutant protein, using Phospho-eIF4G (Ser1108) Antibody. (Provided by Brian Raught, McGill University, Montreal, QuŽbec.)
Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody.
Confocal immunofluorescent analysis of HeLa cells either rapamycin-treated (left) or serum-treated (right), using Phospho-eIF4G (Ser1108) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).