ELAVL1/HuR (D9W7E) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12582
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D9W7E
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1/HuR protein.
• Description: Rabbit Monoclonal
• Gene: ELAVL1

Product images

Western blot analysis of extracts from various cell lines using ELAVL1/HuR (D9W7E) Rabbit mAb.

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ELAVL1/HuR (hELAVL1/HuR-Myc/DDK; +), using ELAVL1/HuR (D9W7E) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of ELAVL1/HuR from 293 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or ELAVL1/HuR (D9W7E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ELAVL1/HuR (D9W7E) Rabbit mAb.

Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or actinomycin D-treated (5 μg/ml, 2 hr; right) using ELAVL1/HuR (D9W7E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow cytometric analysis of HEK-293 cells (green) using ELAVL1/HuR (D9W7E) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alex Fluor® 488 Conjugate) #4412 was used as a secondary antibody.