EML4 (D7Y8F) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12156
• Prices: $277.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7Y8F
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser188 of human EML4 protein.
• Description: Rabbit Monoclonal
• Gene: EML4

Product images

Western blot analysis of extracts from various cell lines using EML4 (D7Y8F) XP ® Rabbit mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® EML4 siRNA (+), using EML4 (D7Y8F) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The EML4 (D7Y8F) XP ® Rabbit mAb confirms silencing of EML4 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Immunoprecipitation of EML4 from 293 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or EML4 (D7Y8F) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EML4 (D7Y8F) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® EML4 siRNA (right), using EML4 (D7Y8F) XP ® Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).