Detection of Human and Mouse by Immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- used at a dilution of 1:5000 (0.2 Detection: DAB.
Detection of Human, Mouse and Rat SMAR1/BANP by Western Blot. Samples: Whole cell lysate (50 ug) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human SMAR1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1 antibody used for IP at 6 ug/mg lysate. SMAR1 was also immunoprecipitated by a previous lot. For blotting immunoprecipitated SMAR1, -2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.