Western Blot analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11.Lane 1: DLX4 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of DLX4 transfected lysate using anti-DLX4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DLX4 rabbit polyclonal antibody.
Detection limit for recombinant GST tagged DLX4 is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DLX4 monoclonal antibody (M01), clone 1F11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.