Sp4 (V-20): sc-645. Western blot analysis of Sp4 expression in NIH/3T3 (A,B) and KNRK (C,D) nuclear extracts.
Sp4 (V-20): sc-645. Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear staining.
Sp4 siRNA (h): sc-36545. Western blot analysis of Sp4 expression in non-transfected control (A) and Sp4 siRNA transfected (B) HeLa cells. Blot probed with Sp4 (V-20): sc-645. α-actinin (H-2): sc-17829 used as specificity and loading control.
Sp4 (V-20): sc-645. ChIP analysis of Sp4 recruitment to genomic amplicons. Four (1-4) different human genomic Sp-1 and two control amplicons (5,6) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Sp4 (V-20): sc-645. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in granular and molecular layers and cytoplasmic staining of Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Sp4 (V-20)-G: sc-645-G. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of cells in germinal center and cells in non-germinal center.