Western blot analysis of extracts from COS-1 cells expressing wild-type or mutant ER alpha, stimulated with beta-estradiol (100 nM) and EGF (100 ng/ml) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser104/106) Antibody (upper) or control ER alpha Antibody #2512 (lower). (Cell lysates provided by Dr. Simak Ali, Hammersmith Hospital, London.)
Western blot analysis of extracts from serum-starved MCF7 cells, untreated, treated with β-estradiol, or treated with β-estradiol and heregulin, using Phospho-Estrogen Receptor α (Ser104/106) Antibody. A non-specific band is detected at 90 kDa.