Western blot (WB) analysis of ACOT8 (P172) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:sp2/0 cell lysate Lane4:H9C2 cell lysate
Immunohistochemistry (IHC) analyzes of ACOT8 (P172) pAb bin paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Western blot (WB) analysis of ACOT8 (P172) pAb at 1:500 dilution Lane1:NIH-3T3 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug)