Western blot analysis of extracts from various cell lines using ETS-1 (D8O8A) Rabbit mAb.
Immunoprecipitation of ETS-1 protein from OVCAR8 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ETS-1 (D8O8A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ETS-1 (D8O8A) Rabbit mAb
Confocal immunofluorescent analysis of OVCAR8 (high expression, left), A549 (moderate expression, center), and HeLa (low expression, right) cells using ETS-1 (D8O8A) Rabbit mAb (green). Actin filaments were labeled with Dylight™ 554 Phalloidin #13054 (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 K-562 cells and either 10 μl of ETS-1 (D8O8A) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human c-Fos Promoter Primers #4663, human c-Myc intron 1 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.