Detection of Human TIP120B by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TIP120B antibody used for IP at 6 ug/mg lysate. TIP120B was also immunoprecipitated by rabbit anti-TIP120B antibody BL14617. For blotting immunoprecipitated TIP120B, was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human and Mouse TIP120B by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-TIP120B antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.