Immunohistochemical of paraffin-embedded H. kidney section using CAPN1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot of lysates from A431, A549, MCF-7, U-251 MG cell line (from left to right) with CAPN1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Flow cytometric of HeLa cells with CAPN1 Antibody (green) compared to an isotype control of mouse IgG1 (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.