Detection of Human CARHSP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CARHSP1 antibody used for IP at 6 ug/mg lysate. CARHSP1 was also immunoprecipitated by rabbit anti-CARHSP1 antibody BL13973. For blotting immunoprecipitated CARHSP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human CARHSP1 by Western Blot. Samples: Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-CARHSP1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.