Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of anti-CASP3 antibody in NCI-H460 cell line lysates (35 ug/lane). CASP3 (arrow) was detected using the purified antibody.
Western blot of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
Flow cytometric of NCI-H460 cells using Caspase-3 (CASP3) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.