Western blot analysis of extracts from various cell lines using OTULIN Antibody.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human OTULIN protein (hOTULIN-Myc/DDK; +) or Myc/DDK-tagged full-length human OTUB1 protein (hOTUB1-Myc/DDK; +), using OTULIN Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower).
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® OTULIN siRNA I #14132 (+), using OTULIN Antibody (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). The OTULIN Antibody confirms silencing of OTULIN expression, while the GAPDH (D16H11) XP ® Rabbit mAb is used as a loading control.
Immunoprecipitation of OTULIN from MCF7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or OTULIN Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using OTULIN Antibody.