Anti-Mouse MIP-1a Antibody - Western Blot. Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E. coli. Recombinant truncated (0.1 ug, 7.8 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.