Detection of Human K by Immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left), breast carcinoma (center) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-K used at a dilution of 1:1000 (1 Detection: DAB.
Detection of Human Cyclin K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.